|
KMID : 1041220130510020142
|
|
Journal of Sericultural and Entomological Science
2013 Volume.51 No. 2 p.142 ~ p.146
|
|
|
Reeling of recombinant flourescence cocoons through low temperature decompressed cooking
|
|
Park Jong-Hwa
Kim Sung-Wan
Jeong Young-Hun
Lee Jong-Kil
Go Young-Mi
Lee Sang-Chan
Choi Kwang-Ho
Kim Seong-Ryul
Goo Tae-Won
|
|
|
Abstract
|
|
|
|
The fluorescent proteins are generally denatured by heat treatment and thus lose their color. The normal reeling method includes processing by drying and cooking the cocoons near 100oC before reeling. Therefore, the usual processing method cannot be used for making colored fluorescent silks. To develop a method that is applicable to producing transgenic silk without color loss, we develop reeling methods adequate for a recombinant fluorescence cocoons. It was found that the fluorescence cocoons keep their native color when dried at temperatures lower than 60oC for 15 h. Also, a new cooking method to soften the fluorescent cocoons was developed: the cocoons were soaked in a solution of 0.2% sodium carbonate (Na2CO3)/0.1% nonionic surfactant (Triton X100) at 60oC and then placed under vacuum. The repeated vacuum treatments enabled complete penetration of the solution into the cocoons, and the cocoons were thus homogenously softened and ready for reeling. In this state, the cooked cocoons can be reeled by an automated reeling machine. Our results suggest that drying and cooking of the cocoons at low temperature enables the subsequent reeling of the colored fluorescent silks by an automatic reeling machine without color loss and can produce silks that can be used for making higher value-added silk materials.
|
|
|
KEYWORD
|
|
|
Fluorescence cocoon, Reeling, Silkworm, Transgenesis
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|
|